epatocytes Differentiation from iPSC
With decades of experience in the field of iPSCs differentiation, Creative Biolabs has been a leading service provider of iPSC-derived differentiated cell in multiple types. Especially, we have extensive experience in producting neuronal cells, hepatocytes, and cardiomyocytes. Now we offer the service of hepatocytes differentiation from iPSC to our clients all over the world.
Introduction of iPSC Derived Hepatocytes
Liver is regarded as an important organ in mamal as it plays a crucial role in protein synthesis, detoxification, metabolism and excretion. Therefore, the generation of healthy hepatocytes is a major goal for hepatic disease therapy. As human induced pluripotent stem cells (hiPSCs) have the potential to differentiat into different cell types, it is possible to generate iPSC-derived hepatocytes for a wide range of applications. Now Creative Biolabs is able to provide hepatocyte differentiation service from iPSC.
Processes of Hepatocytes Differentiation from iPSC
Generation of iPSC derived hepatocytes has been served as the potential source for therapeutic applications and enables in vitro study of liver diseases. During embryogenesis, the differentiation processes have been understood well by the comprehensive of molecular correlates in liver development. In mammalian embryos, the ventral foregut endoderm is the tissue from which liver originates. In this case, there are three essential consecutive processes for the generation of hepatocytes which further differentiated into definitive endoderm, hepatic progenitors, and mature hepatocytes. The initial step is the phenotype generation of definitive endoderm which requires exposure to the transforming growth factor b (TGF-b) superfamily members activin A and bone morphogenic protein 4 (BMP4). In addition, short-term exposure to Wnt3a, which is expressed in key stages of human liver development and specifically interacting with activin A, enhances the production of both definitive and hepatic endoderm. The combination of fibroblast growth factors and BMP4 contributes largely to embryonic development. We also found that the presence of fetal bovine serum in medium inhibits some factors for early mammalian embryo development. The second step is the generation of hepatic progenitor cells from definitive endoderm which is accomplished by adding specific factors which includes hepatocyte growth factor (HGF) and hepatocyte nuclear factor 4 alpha (HNF4a). The final step is the induction of hepatocyte maturation via adding oncostatin M (OSM), an interleukin-6 family cytokine, in combination with glucocorticoids in the cell culture medium.
