With years of experience and high-end technologies, Creative Biolabs has successfully developed a series of innovative and diversified assay platforms to provide fast and convenient services for our customers. We offer high-quality genetic/epigenetic analysis services for customers all over the world.
Background of Genetic/epigenetic
Epigenetics refers to the reversible regulation of gene expression, independent of the occurrence of DNA sequences. Epigenetic modifications, mediated by DNA methylation, histone tail modifications, and non-coding RNA interventions, make the genome susceptible to malignant progression. Chromatin remodeling by epigenetic reprogramming controls the regulation of gene expression and has important implications in the development of human cancers. Epigenetic mechanisms allow cells to alter their transcriptional activity, thereby permitting changes in gene expression without altering DNA sequence. There is also an increasing evidence for epigenetic modulation in response to environmental factors, and its implication in several human diseases such as cancer, neurodegenerative diseases, and cardiovascular diseases has been well established. Epigenetics can be triggered by any environmental signal, including UV, atmospheric pressure, food supply, plant nutrition, water supply, oxygen, chemicals, temperature and climate change.
Massively parallel sequencing technology lays the foundation for the construction of epigenomics. Creative Biolabs provides key sequencing-based methods used in the analysis of epigenomes.
- Bisulfite Sequencing
5-methyl-cytosine (5mC) and its oxidized derivatives are measured on a genome-wide basis using enrichment and transformation methods followed by massively parallel sequencing. Bisulfite conversion provides a quantitative measurement of 5mC but does not distinguish 5hmC. Antibody enrichment provides qualitative measurements of 5mC and 5hmC. The bisulfite converted or enriched DNA is purified, library constructed and clonally sequenced. A special algorithm is needed to align the bisulfite conversion readings with the reference genome.