At Creative Biolabs, we enhance your research and data relevancy by acquiring and reprogramming induced pluripotent stem cells (iPSC) from a specific patient clinical profile. Our pipeline is designed to meet the clients’ project and specific assay requirements for cellular characteristics and functions.
iPSC Reprogramming Service
Direct reprogramming of somatic cells into induced pluripotent stem (iPS) cells is a valuable method to produce patient-specific stem cells of any lineage without the use of embryonic materials. Various new strategies have been developed to improve the reprogramming technologies since the first report of iPS cells generation from murine fibroblasts using retroviral transduction of a defined set of transcription factors. Methods of factor reprogramming fall into two broad categories: chemical and transgene reprogramming. It has been reported that many small molecules promote reprogramming when used with classical reprogramming factors. Creative Biolabs has developed streamed-line protocols for efficient iPSC generation with viral vectors, DNA (plasmid), RNA and recombinant proteins. Each of our services will be provided with a comprehensive report suitable for publications.
- Retrovirus vector
- Lentivirus vector
- Adenovirus vector
- Sendai virus vector
- PiggyBac transposon vector
- Episomal plasmid vector
- Direct delivery of synthetic mRNAs.
- Mature double-stranded miRNAs
- Factor Delivery by Protein
The primary factors to consider when deciding on a reprogramming method to generate iPSCs are what cell is being reprogrammed, the ability of the reprogramming method to adequately reprogram this cell type, as well as whether the presence of integrated sequences in the iPSCs will hinder downstream application.
